Advance in Methodology and Strategies To Unveil Metabolic Mechanisms of Pesticide Residues in Food Crops.

Pesticide residues are a food safety concern. A good detection method is critical for rapid and accurate determination of pesticide metabolites in crops and studying metabolism. The pretreatment methods have mainly been ultrasonic extraction-solid-phase extraction and QuEChERS, while detection methods have been radio-chromatography, nuclear magnetic resonance, and mass spectrometry. This perspective briefed the progress of analytical methods used for studying pesticide transformation in crops over the past decade. With the combination of the characteristics of the pesticide molecular structure and the transformation principles of pesticides in crops, we presented specific methods for elucidating new metabolites and the approaches to identify metabolites using multi-high-resolution mass spectrometry.