Login / Signup

Establishment and characterization of an immortalized epicardial cell line.

Haobin JiangShen SongJiacheng LiQianqian YinShengshou HuNie Yu
Published in: Journal of cellular and molecular medicine (2021)
Recently, the increasing significance of the epicardium in cardiac development and regeneration is beginning to be recognized. However, because of the small proportion of primary epicardial cells and the limited cell culture time, further research on the mechanism of epicardial cells is hindered. Here, we transfected simian virus 40 Large T (SV40-LT) into primary epicardial cells to establish an immortalized cell line, named EpiSV40. We further demonstrated that EpiSV40 can be easy to culture and has the proliferation, migration and differentiation capacities comparable to primary epicardial cells. EpiSV40 can serve as an ideal in vitro model for epicardial cell research, which will booster the study of the epicardium in cardiac development and heart regeneration.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • stem cells
  • signaling pathway
  • endoplasmic reticulum stress
  • cell death
  • atrial fibrillation
  • left ventricular
  • oxidative stress
  • mesenchymal stem cells
  • bone marrow
  • pi k akt