Ordered immobilization of serine proteases enabled by a linchpin directed modification platform.

Dattatraya Gautam RawaleMrityunjay GuptaKalyani ThakurRagendu VVishal Rai

Published in: Chemical communications (Cambridge, England) (2024)

We report a chemoselective and site-selective precision engineering of lysine in proteases. The mild and physiological reaction conditions keep their auto-degradation under control. Furthermore, it enables single-site ordered immobilization, enhancing protein digestion and peptide mapping efficiency.

Keyphrases

high resolution
magnetic nanoparticles
high density
binding protein
anaerobic digestion
single cell