Login / Signup

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining.

Philip Seifert
Published in: Journal of histotechnology (2024)
The HistoEnder, an inexpensive open-source 3D printer published as an automated histological slide stainer, has been adapted for conventional biological transmission electron microscopy (TEM) batch grid staining. Details are presented of the 3D printed apparatus, assembly, G-code programming, and operation on the 3D printer to post-section stains up to 20 grids through aqueous uranyl acetate, distilled water rinses, and lead stains. TEM Results are identical to manual staining with the advantages of automation using the low cost HistoEnder, apparatus, and equipment.
Keyphrases
  • electron microscopy
  • low cost
  • flow cytometry
  • machine learning
  • deep learning
  • high throughput
  • single cell