Biocompatibility Computation of Muscle Cells on Polyhedral Oligomeric Silsesquioxane-Grafted Polyurethane Nanomatrix.
Touseef AmnaMallick Shamshi HassanMohamed H El-NewehyTariq AlghamdiMeera Moydeen Abdul HameedMyung-Seob KhilPublished in: Nanomaterials (Basel, Switzerland) (2021)
This study was performed to appraise the biocompatibility of polyhedral oligomeric silsesquioxane (POSS)-grafted polyurethane (PU) nanocomposites as potential materials for muscle tissue renewal. POSS nanoparticles demonstrate effectual nucleation and cause noteworthy enhancement in mechanical and thermal steadiness as well as biocompatibility of resultant composites. Electrospun, well-aligned, POSS-grafted PU nanofibers were prepared. Physicochemical investigation was conducted using several experimental techniques, including scanning electron microscopy, energy dispersive X-ray spectroscopy, electron probe microanalysis, Fourier transform infrared spectroscopy, and X-ray diffraction pattern. Adding POSS molecules to PU did not influence the processability and morphology of the nanocomposite; however, we observed an obvious mean reduction in fiber diameter, which amplified specific areas of the POSS-grafted PU. Prospective biomedical uses of nanocomposite were also appraised for myoblast cell differentiation in vitro. Little is known about C2C12 cellular responses to PU, and there is no information regarding their interaction with POSS-grafted PU. The antimicrobial potential, anchorage, proliferation, communication, and differentiation of C2C12 on PU and POSS-grafted PU were investigated in this study. In conclusion, preliminary nanocomposites depicted superior cell adhesion due to the elevated free energy of POSS molecules and anti-inflammatory potential. These nanofibers were non-hazardous, and, as such, biomimetic scaffolds show high potential for cellular studies and muscle regeneration.
Keyphrases
- electron microscopy
- tissue engineering
- reduced graphene oxide
- high resolution
- skeletal muscle
- cell adhesion
- human health
- quantum dots
- staphylococcus aureus
- signaling pathway
- induced apoptosis
- anti inflammatory
- healthcare
- gold nanoparticles
- solid phase extraction
- magnetic resonance
- cell cycle arrest
- cell death
- climate change
- risk assessment
- computed tomography
- single molecule
- wound healing
- social media
- atomic force microscopy
- living cells
- solid state