Macrophage-Laden Gold Nanoflowers Embedded with Ultrasmall Iron Oxide Nanoparticles for Enhanced Dual-Mode CT/MR Imaging of Tumors.
Yucheng PengXiaomeng WangYue WangYue GaoRui GuoXiangyang ShiXueyan CaoPublished in: Pharmaceutics (2021)
The design of multimodal imaging nanoplatforms with improved tumor accumulation represents a major trend in the current development of precision nanomedicine. To this end, we report herein the preparation of macrophage (MA)-laden gold nanoflowers (NFs) embedded with ultrasmall iron oxide nanoparticles (USIO NPs) for enhanced dual-mode computed tomography (CT) and magnetic resonance (MR) imaging of tumors. In this work, generation 5 poly(amidoamine) (G5 PAMAM) dendrimer-stabilized gold (Au) NPs were conjugated with sodium citrate-stabilized USIO NPs to form hybrid seed particles for the subsequent growth of Au nanoflowers (NFs). Afterwards, the remaining terminal amines of dendrimers were acetylated to form the dendrimer-stabilized Fe3O4/Au NFs (for short, Fe3O4/Au DSNFs). The acquired Fe3O4/Au DSNFs possess an average size around 90 nm, display a high r1 relaxivity (1.22 mM-1 s-1), and exhibit good colloidal stability and cytocompatibility. The created hybrid DSNFs can be loaded within MAs without producing any toxicity to the cells. Through the mediation of MAs with a tumor homing and immune evasion property, the Fe3O4/Au DSNFs can be delivered to tumors more efficiently than those without MAs after intravenous injection, thus significantly improving the MR/CT imaging performance of tumors. The developed MA-mediated delivery system may hold great promise for enhanced tumor delivery of other contrast agents or nanomedicines for precision cancer nanomedicine applications.
Keyphrases
- contrast enhanced
- iron oxide nanoparticles
- computed tomography
- magnetic resonance
- sensitive detection
- magnetic resonance imaging
- reduced graphene oxide
- dual energy
- image quality
- positron emission tomography
- high resolution
- induced apoptosis
- drug delivery
- cancer therapy
- squamous cell carcinoma
- high dose
- pain management
- silver nanoparticles
- machine learning
- gold nanoparticles
- depressive symptoms
- big data
- low dose
- cell death
- cell cycle arrest
- oxidative stress
- artificial intelligence
- chronic pain