Comparison of Immunohistochemistry and Direct Sanger Sequencing for Detection of the BRAF(V600E) Mutation in Thyroid Neoplasm.

Hye Seon OhHyemi KwonSuyeon ParkMijin KimMin Ji JeonTae Yong KimYoung Kee ShongWon Bae KimJene ChoiWon Gu Kim Dong Eun Song

Published in: Endocrinology and metabolism (Seoul, Korea) (2018)

IHC could be an alternative method to direct Sanger sequencing for BRAF(V600E) mutation detection both in postoperative and preoperative samples. However, application of IHC to detect the BRAF(V600E) mutation in FNA samples is of limited value compared with direct sequencing.

Keyphrases

single cell
metastatic colorectal cancer
patients undergoing
loop mediated isothermal amplification
wild type
real time pcr
label free
low grade