Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Magalie MartineauAgila SomasundaramJonathan B GrimmTodd D GruberDaniel ChoquetJustin W TaraskaLuke D LavisDavid PerraisPublished in: Nature communications (2017)
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.