KRAS mutations by digital PCR in circulating tumor cells isolated from the mesenteric vein are associated with residual disease and overall survival in resected colorectal cancer patients.
Yan LiMariano MonzoIsabel MorenoFrancisco Martinez-RodenasRaquel HernandezJoan J CastellanoJordi CanalsBing HanCarmen MuñozAlfons NavarroPublished in: International journal of colorectal disease (2020)
DGPCR is more efficient than Sanger sequencing for detecting KRAS mutations. KRAS G13D mutations and high mutant KRAS copy number are associated with shorter OS. The analysis of KRAS mutations in CTCs from blood obtained at the time of surgery can identify patients with a higher risk of relapse.
Keyphrases
- wild type
- circulating tumor cells
- copy number
- mitochondrial dna
- end stage renal disease
- prognostic factors
- chronic kidney disease
- ejection fraction
- newly diagnosed
- minimally invasive
- genome wide
- dna methylation
- circulating tumor
- peritoneal dialysis
- single cell
- coronary artery bypass
- acute coronary syndrome
- cell free