Photothermal Intracellular Delivery Using Gold Nanodisk Arrays.
Chuanzhen ZhaoTianxing ManXiaobin XuQing YangWenfei LiuSteven J JonasMichael A TeitellPei-Yu ChiouPaul S WeissPublished in: ACS materials letters (2020)
Local heating using pulsed laser-induced photothermal effects on plasmonic nanostructured substrates can be used for intracellular delivery applications. However, the fabrication of plasmonic nanostructured interfaces is hampered by complex nanomanufacturing schemes. Here, we demonstrate the fabrication of large-area plasmonic gold (Au) nanodisk arrays that enable photothermal intracellular delivery of biomolecular cargo at high efficiency. The Au nanodisks (350 nm in diameter) were fabricated using chemical lift-off lithography (CLL). Nanosecond laser pulses were used to excite the plasmonic nanostructures, thereby generating transient pores at the outer membranes of targeted cells that enable the delivery of biomolecules via diffusion. Delivery efficiencies of >98% were achieved using the cell impermeable dye calcein (0.6 kDa) as a model payload, while maintaining cell viabilities at >98%. The highly efficient intracellular delivery approach demonstrated in this work will facilitate translational studies targeting molecular screening and drug testing that bridge laboratory and clinical investigations.
Keyphrases
- highly efficient
- cancer therapy
- photodynamic therapy
- single molecule
- high efficiency
- single cell
- reactive oxygen species
- emergency department
- sensitive detection
- energy transfer
- cell proliferation
- mass spectrometry
- mesenchymal stem cells
- brain injury
- electronic health record
- subarachnoid hemorrhage
- drug induced
- signaling pathway
- high speed
- silver nanoparticles