Selective fragmentation of the N-glycan moiety and protein backbone of ribonuclease B on an Orbitrap Fusion Lumos Tribrid mass spectrometer.
Shasha LiYue ZhouKaijie XiaoJing LiZhixin TianPublished in: Rapid communications in mass spectrometry : RCM (2018)
For comprehensive structural characterization of intact N-glycoproteins using tandem mass spectrometry, the composition and topology of the N-glycan moiety can be identified using HCD and EThcD complementarily at low and high energies; while the amino acid sequence and glycosite can be identified using CID, ETD, HCD, ETciD, and EThcD with their optimal dissociation parameters.
Keyphrases
- tandem mass spectrometry
- amino acid
- ultra high performance liquid chromatography
- high resolution
- liquid chromatography
- high performance liquid chromatography
- gas chromatography
- simultaneous determination
- high resolution mass spectrometry
- mass spectrometry
- cell surface
- solid phase extraction
- density functional theory
- binding protein