Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography-Mass Spectrometry Analysis of Polypeptide.
Zuzana ZajickovaLucie NovákováFrantišek ŠvecPublished in: Analytical chemistry (2020)
The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography-mass spectrometry (SFC-MS). We demonstrate for the first time the SFC-MS separation of a mixture of polypeptides carried out using poly(styrene-co-divinylbenzene) monolithic columns and carbon dioxide/methanol mobile phase. A gradient from 2 to 40% methanol modifier containing 0.1% TFA as an acidic additive was applied for the optimized elution and the separation was achieved in less than 3 min. Selected ion monitoring enabled detection of selected masses characteristic of three ionophoric pentadecapeptide antibiotics gramicidin A, B, and C and their two corresponding isoforms. Furthermore, their identity was confirmed through determination of their [M + 2H]2+, [M + 2Na]2+, and [M + H + Na]2+ ions acquired by positive-ion electrospray ionization-mass spectrometry (ESI-MS).
Keyphrases
- liquid chromatography
- mass spectrometry
- carbon dioxide
- solid phase extraction
- tandem mass spectrometry
- high performance liquid chromatography
- gas chromatography
- capillary electrophoresis
- simultaneous determination
- ms ms
- high resolution
- molecularly imprinted
- magnetic resonance imaging
- ionic liquid
- multiple sclerosis
- quantum dots
- water soluble
- magnetic resonance
- amino acid
- high speed