Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor.
Mohamed Amine BerkalJean-Jacques ToulmeCorinne NardinPublished in: Analytical and bioanalytical chemistry (2024)
Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R 2 ) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.
Keyphrases
- label free
- human health
- loop mediated isothermal amplification
- sensitive detection
- risk assessment
- quantum dots
- climate change
- escherichia coli
- real time pcr
- single molecule
- energy transfer
- living cells
- magnetic resonance imaging
- high resolution
- gold nanoparticles
- hydrogen peroxide
- machine learning
- deep learning
- solid phase extraction
- candida albicans
- molecularly imprinted