Attenuating Fibrotic Markers of Patient-Derived Dermal Fibroblasts by Thiolated Lignin Composites.
Jorge A BelgodereDongwan SonBokyoung JeonJongwon ChoeAnna C GuidryAdam X BaoSyed A ZaminUmang M ParikhSwathi BalajiMyungwoong KimJangwook P JungPublished in: ACS biomaterials science & engineering (2021)
We report the use of phenolic functional groups of lignosulfonate to impart antioxidant properties and the cell binding domains of gelatin to enhance cell adhesion for poly(ethylene glycol) (PEG)-based scaffolds. Chemoselective thiol-ene chemistry was utilized to form composites with thiolated lignosulfonate (TLS) and methacrylated fish gelatin (fGelMA). Antioxidant properties of TLS were not altered after thiolation and the levels of antioxidation were comparable to those of L-ascorbic acid. PEG-fGelMA-TLS composites significantly reduced the difference in COL1A1, ACTA2, TGFB1, and HIF1A genes between high-scarring and low-scarring hdFBs, providing the potential utility of TLS to attenuate fibrotic responses.
Keyphrases
- cell adhesion
- reduced graphene oxide
- tissue engineering
- oxidative stress
- systemic sclerosis
- drug delivery
- idiopathic pulmonary fibrosis
- anti inflammatory
- hyaluronic acid
- single cell
- visible light
- cell therapy
- genome wide
- extracellular matrix
- aqueous solution
- bone regeneration
- ionic liquid
- endothelial cells
- drug discovery
- mesenchymal stem cells
- bioinformatics analysis
- binding protein
- genome wide identification