Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. The Baker's Asthma Case.
Fatma BoukidBarbara PrandiStefano SforzaRhouma SayarYong Weon SeoMondher MejriInes YacoubiPublished in: Journal of agricultural and food chemistry (2017)
Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.
Keyphrases
- chronic obstructive pulmonary disease
- climate change
- allergic rhinitis
- lung function
- protein protein
- ms ms
- amino acid
- multiple sclerosis
- genome wide
- binding protein
- mass spectrometry
- electronic health record
- copy number
- computed tomography
- machine learning
- magnetic resonance imaging
- nitric oxide
- single cell
- big data
- artificial intelligence
- transcription factor
- diffusion weighted imaging