Alzheimer's disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for the point-of-care detection of AD biomarkers in the blood. Such a portable platform integrates nanoAu-modified vertical graphene (VG@Au) into a working electrode, which can significantly improve sensitivity and reduce detection limit due to the large specific surface, excellent electrical conductivity, high stability, and good biocompatibility. The tau protein, as an important factor in the course of AD, is selected as a key AD biomarker. The results show that the linear range of this sensing platform is 0.1 pg/mL to 1 ng/mL, with a detection limit of 0.034 pg/mL (S/N = 3), indicating that this portable sensing platform meets the demand for the detection of the tau protein in the blood. This work offers great potential for AD diagnosis and personal healthcare.
Keyphrases
- healthcare
- label free
- high throughput
- loop mediated isothermal amplification
- real time pcr
- gold nanoparticles
- low cost
- protein protein
- public health
- cerebrospinal fluid
- endothelial cells
- mental health
- amino acid
- risk assessment
- binding protein
- health information
- ionic liquid
- room temperature
- carbon nanotubes
- small molecule
- social media
- molecularly imprinted
- single cell
- health promotion