Biomimetic Hydroxyapatite Crystals Growth on Phosphorylated Chitosan Films by In Vitro Mineralization Used as Dental Substitute Materials.
Fathia RahmaniOmar Larbi BouamraneAmina Ben BouabdallahLeonard Ionuţ AtanaseAbdelkader HellalAurelian Nichita ApintilieseiPublished in: Polymers (2023)
Chitosan (CS) films exhibit great potential as a substrate for the in vitro mineralization process. In this study, to mimic the formation of nanohydroxyapatite (HAP) as natural tissue, CS films coated with a porous calcium phosphate were investigated using scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDX), Fourier transforms infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and X-ray photoelectron spectroscopy (XPS). Calcium phosphate coating deposited on phosphorylated derivatives of CS was obtained by a process based on phosphorylation, Ca(OH) 2 treatment and artificial saliva solution (ASS) immersion. The phosphorylated CS films (PCS) were obtained by partial hydrolysis of the PO 4 functionalities. It was demonstrated that this precursor phase could induce the growth and the nucleation of the porous calcium phosphate coating when immersed in ASS. Moreover, oriented crystals and qualitative control of calcium phosphate phases on CS matrices are obtained in a biomimetic mode. Furthermore, in vitro antimicrobial activity of PCS was evaluated against three species of oral bacteria and fungi. It revealed an increase in antimicrobial activity with minimum inhibition concentration (MIC) values of 0.10% ( Candida albicans ), 0.05% ( Staphylococcus aureus) and 0.025% ( Escherichia coli ) which proves their possible use as dental substitute materials.
Keyphrases
- electron microscopy
- room temperature
- high resolution
- candida albicans
- escherichia coli
- staphylococcus aureus
- biofilm formation
- tissue engineering
- ionic liquid
- drug delivery
- oral health
- mass spectrometry
- dual energy
- single molecule
- systematic review
- wound healing
- carbon nanotubes
- magnetic resonance
- solid phase extraction
- klebsiella pneumoniae
- computed tomography
- liquid chromatography
- anaerobic digestion