Microbiological investigation of pregnancies following vaginal radical trachelectomy using 16S rRNA sequencing of FFPE placental specimens.
Risa TsunematsuTasuku MariyaMina UmemotoShiori OgawaWataru AraiSuguru E TanakaKyota AshikawaTerufumi KuboYoshiyuki SakurabaTsuyoshi BabaShinichi IshiokaToshiaki EndoTsuyoshi SaitoPublished in: FEBS open bio (2024)
This study examined the risk of intrauterine infection associated with radical trachelectomy (RT) in early-stage cervical cancer patients. This procedure preserves fertility but is linked to increased risk of intrauterine infection due to cervical defects during pregnancy. DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) placental specimens of 23 pregnant post-RT patients and 16S rRNA gene sequencing was used for bacterial identification. The prevalence of Lactobacillus crispatus and Burkholderia stabilis was significantly higher in the non-chorioamnionitis group. In contrast, alpha diversity analysis using the PD index showed significantly higher diversity in the chorioamnionitis group (P = 0.04). The demonstrated relationship between chorioamnionitis and microbial diversity affirms the importance of controlling the genital bacterial flora in pregnancies following RT.
Keyphrases
- early stage
- end stage renal disease
- preterm birth
- ejection fraction
- newly diagnosed
- magnetic resonance
- chronic kidney disease
- single cell
- microbial community
- gestational age
- pregnant women
- peritoneal dialysis
- risk factors
- prognostic factors
- circulating tumor
- magnetic resonance imaging
- cell free
- gene expression
- copy number
- squamous cell carcinoma
- computed tomography
- radiation therapy
- patient reported
- neoadjuvant chemotherapy
- bioinformatics analysis