hnRNP-K Targets Open Chromatin in Mouse Embryonic Stem Cells in Concert with Multiple Regulators.
Evgeny I BakhmetIgor B NazarovAdel R GazizovaNadezhda E VorobyevaAndrey A KuzminMikhail N GordeevSergey A SinenkoNikolai D AksenovTatyana O ArtamonovaMikhail A KhodorkovskiiNatalia AleninaDaria OnichtchoukGuangming WuHans R SchölerAlexey N TomilinPublished in: Stem cells (Dayton, Ohio) (2019)
The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018-1029.
Keyphrases
- embryonic stem cells
- transcription factor
- genome wide
- binding protein
- gene expression
- dna methylation
- stem cells
- optical coherence tomography
- dna binding
- diabetic retinopathy
- dna damage
- minimally invasive
- cell therapy
- signaling pathway
- high throughput
- optic nerve
- genome wide identification
- drug delivery
- oxidative stress
- circulating tumor
- rna seq
- drug induced
- metal organic framework