A NIR-emitting cyanine with large Stokes shifts for live cell imaging: large impact of the phenol group on emission.

Dipendra DahalLucas McDonaldSabita PokhrelSailaja ParuchuriMichael C Konopka Yi Pang

Published in: Chemical communications (Cambridge, England) (2019)

There are a limited number of near-infrared (NIR) emitting (λem = 700-900 nm) molecular probes for imaging applications. A NIR-emitting probe that exhibits emission at ∼800 nm with a large Stokes shift was synthesized and found to exhibit excellent selectivity towards mitochondria for live-cell imaging. The photophysical properties were attributed to an excited "cyanine structure" via intramolecular charge transfer (ICT) involving a phenol group.

Keyphrases

fluorescent probe
living cells
photodynamic therapy
fluorescence imaging
high resolution
quantum dots
energy transfer
drug release
small molecule
cell death
reactive oxygen species
endoplasmic reticulum