High-throughput sequencing of peripheral blood for minimal residual disease monitoring in childhood precursor B-cell acute lymphoblastic leukemia: A prospective feasibility study.
Jack BartramGary WrightStuart AdamsPaul ArcherTony BrooksDarren EdwardsJerry HancockHenrik KnechtSarah InglottEdward MountjoyMarie RoynaneStephanie WakemanJohn MoppettMike HubankNick GouldenPublished in: Pediatric blood & cancer (2021)
This is proof of principle that using the increased sensitivity of HTS, PB can be used to measure MRD and stratify children with ALL. The method is cost effective, rapid, accurate, and reproducible, with inherent advantages in children. Importantly, increasing the frequency testing by PB as opposed to intermittent BM sampling may allow extension of the dynamic range of MRD, giving a more complete picture of the kinetics of disease remission while improving relapse prediction and speed of detection.
Keyphrases
- acute lymphoblastic leukemia
- peripheral blood
- high throughput sequencing
- young adults
- heavy metals
- loop mediated isothermal amplification
- aqueous solution
- high resolution
- allogeneic hematopoietic stem cell transplantation
- high intensity
- risk assessment
- free survival
- real time pcr
- disease activity
- acute myeloid leukemia
- sensitive detection
- quantum dots