Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae.

Raquel M CadeteAlejandro M de Las HerasAnders G SandströmCarla FerreiraFrancisco GírioMarie-Françoise Gorwa-GrauslundCarlos A RosaCésar Fonseca

Published in: Biotechnology for biofuels (2016)

This work explored a new clade of xylose-fermenting yeasts (Spathaspora species) towards the engineering of S. cerevisiae for improved xylose fermentation. The new S. cerevisiae TMB 3504 displays higher XR activity with NADH than with NADPH, with consequent improved ethanol yield and productivity and low xylitol production. This meaningful advance in anaerobic xylose fermentation by recombinant S. cerevisiae (using the XR/XDH pathway) paves the way for the development of novel industrial pentose-fermenting strains.

Keyphrases

saccharomyces cerevisiae
wastewater treatment
microbial community
climate change
heavy metals
sewage sludge
anaerobic digestion