TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2.

Eduardo Pinho MeloCarlos LopesPeter GollwitzerStephan LortzSigurd LenzenIlir MehmetiClemens F KaminskiDavid RonEdward Avezov

Published in: BMC biology (2017)

A tri-cysteine system with a single peroxidatic thiol enables H2O2 detection in oxidizing milieux such as that of the ER. Tracking ER H2O2 in live pancreatic β-cells points to a role for glutathione in H2O2 turnover.

Keyphrases

endoplasmic reticulum
induced apoptosis
living cells
cell cycle arrest
high resolution
fluorescent probe
endoplasmic reticulum stress
high speed
loop mediated isothermal amplification
signaling pathway
mass spectrometry
cell proliferation