A simplified method for the efficient purification and refolding of recombinant human TRAIL.
Zhenjiang ZhangSu Bin HahnThong M CaoMichael R KingPublished in: Biotechnology progress (2020)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) belongs to the TNF cytokine superfamily that specifically induces apoptosis in a broad spectrum of human cancer cell lines but not in most healthy cells. The antitumor potential of recombinant human TRAIL (rhTRAIL) has attracted great attention among biologists and oncologists. However, attempts to express rhTRAIL in Escherichia coli often results in limited yield of bioactive protein due to the formation of inclusion bodies (IBs), which are dense insoluble particulate protein aggregates inside cells. We describe herein a highly simplified method to produce pure bioactive rhTRAIL using E. coli. The method is straightforward and requires only basic laboratory equipment, with highly efficient purification and high yield of renaturation, and may also be applied to produce other proteins that form IBs in E. coli.
Keyphrases
- recombinant human
- escherichia coli
- cell cycle arrest
- induced apoptosis
- highly efficient
- rheumatoid arthritis
- endoplasmic reticulum stress
- cell death
- oxidative stress
- endothelial cells
- protein protein
- pi k akt
- young adults
- amino acid
- staphylococcus aureus
- induced pluripotent stem cells
- risk assessment
- cystic fibrosis
- multidrug resistant
- candida albicans