Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS.
Yunshuang FanYamei LiYuanyuan WuLixin LiYuming WangYu-Bo LiPublished in: Evidence-based complementary and alternative medicine : eCAM (2017)
Simiao Wan (SMW), an important multiherbal formula used in traditional Chinese medicine, is extensively used to treat rheumatoid arthritis. However, the knowledge of the bioactive components of SMW remains unclear. Thus, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were used to analyze the chemical constituents of volatile and nonvolatile extracts of SMW, as well as its absorbed components in rat plasma after oral SMW administration. Identification of several compounds was enabled by comparison of retention times, MS spectra, and MS/MS spectral data with the standard substance and reference materials reported in the literature. In the volatile extracts, GC-MS identified 26 compounds in vitro, three of which observed in blood by GC-MS. In the nonvolatile extracts, LC-MS identified 49 compounds in SMW; 18 compounds containing 7 prototype compounds, 5 metabolites, and 6 unknown compounds were absorbed by blood. The proposed GC-MS and LC-MS method was appropriate not only for the rapid screening and identification of multiple components of an SMW extract but also for screening its bioactive constituents in vivo. The proposed method could be a promising tool for the quality control of other Chinese herbal medicines.
Keyphrases
- mass spectrometry
- ms ms
- liquid chromatography
- gas chromatography
- rheumatoid arthritis
- gas chromatography mass spectrometry
- quality control
- healthcare
- magnetic resonance imaging
- tandem mass spectrometry
- oxidative stress
- high performance liquid chromatography
- high resolution
- multidrug resistant
- magnetic resonance
- liquid chromatography tandem mass spectrometry
- interstitial lung disease
- contrast enhanced
- anti inflammatory
- essential oil