Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis.
Tatsuhiko OzawaEiji KobayashiHiroshi HamanaTomoko NakamuraFulian LyuAtsushi HayashiAtsushi MuraguchiHiroyuki KishiPublished in: European journal of immunology (2021)
Generation of TCR-like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip-based Ab-secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single-cell level to obtain TCR-like antibodies; blocking procedure enables us to avoid the detection of non-TCR-like antibodies.