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Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe.

Ingrid CipakovaMatus JurcikVeronika RubintovaMarianna BorbovaBarbora MikolaskovaJan JurcikJana BellovaPeter BaráthJuraj GreganLubos Cipak
Published in: Cell cycle (Georgetown, Tex.) (2019)
The spliceosome is a complex molecular machine assembled from many components, which catalyzes the removal of introns from mRNA precursors. Our previous study revealed that the Nrl1 (NRDE-2 like 1) protein associates with spliceosome proteins and regulates pre-mRNA splicing and homologous recombination-dependent R-loop formation in the fission yeast Schizosaccharomyces pombe. Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals additional insights into the protein interaction network of the spliceosome. We discuss implications of these findings in the light of recent progress in our understanding of how Nrl1 and splicing factors ensure genome stability.
Keyphrases
  • binding protein
  • dna repair
  • amino acid
  • deep learning
  • transcription factor
  • oxidative stress
  • single molecule
  • dna methylation
  • single cell
  • genome wide