Rapid Detection and Quantification of Patulin and Citrinin Contamination in Fruits.
Sudharsan SadhasivamOmer BardaVarda ZakinRam ReifenEdward SionovPublished in: Molecules (Basel, Switzerland) (2021)
Patulin (PAT) and citrinin (CTN) are the most common mycotoxins produced by Penicillium and Aspergillus species and are often associated with fruits and fruit by-products. Hence, simple and reliable methods for monitoring these toxins in foodstuffs are required for regular quality assessment. In this study, we aimed to establish a cost-effective method for detection and quantification of PAT and CTN in pome fruits, such as apples and pears, using high-performance liquid chromatography (HPLC) coupled with spectroscopic detectors without the need for any clean-up steps. The method showed good performance in the analysis of these mycotoxins in apple and pear fruit samples with recovery ranges of 55-97% for PAT and 84-101% for CTN, respectively. The limits of detection (LOD) of PAT and CTN in fruits were 0.006 µg/g and 0.001 µg/g, while their limits of quantification (LOQ) were 0.018 µg/g and 0.003 µg/g, respectively. The present findings indicate that the newly developed HPLC method provides rapid and accurate detection of PAT and CTN in fruits.
Keyphrases
- liquid chromatography
- solid phase extraction
- high performance liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- loop mediated isothermal amplification
- simultaneous determination
- label free
- real time pcr
- high resolution
- sensitive detection
- molecular dynamics simulations
- molecular docking
- ms ms
- human health
- cell wall