Anti-Cancer and Anti-Inflammatory Potential of the Green Synthesized Silver Nanoparticles of the Red Sea Sponge Phyllospongia lamellosa Supported by Metabolomics Analysis and Docking Study.
Areej A Al-KhalafHossam M HassanAisha M AlrajhiRania Ali El Hadi MohamedWael N HozzeinPublished in: Antibiotics (Basel, Switzerland) (2021)
We prepared a chloroform extract (CE) and ethyl acetate extract (EE) of the Red Sea sponge Phyllospongia lamellosa synthesized silver nanoparticles. The prepared silver nanoparticles were characterized through UV-vis spectrophotometric, transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR) analyses. Testing for their anti-cancer activities was performed against MCF-7, MDB-231, and MCF-10A cells. Anti-inflammatory activity against COX-1 and 2 was assessed. Furthermore, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics analysis and molecular docking were also applied.
Keyphrases
- silver nanoparticles
- mass spectrometry
- liquid chromatography
- molecular docking
- anti inflammatory
- molecular dynamics simulations
- oxidative stress
- electron microscopy
- simultaneous determination
- high resolution mass spectrometry
- high resolution
- breast cancer cells
- high performance liquid chromatography
- gas chromatography
- tandem mass spectrometry
- risk assessment
- cell cycle arrest
- cell death
- molecular dynamics
- ionic liquid
- ms ms