Sensitivity comparison for the Leishmania spp. detection in different canine tissues using PCR-HRM.

Ana Fidelina Gómez Garay Stefania Fraenkel Jorge Javier Alfonso Ruiz Diaz Oscar Daniel Salvioni Recalde María Celeste Vega Gómez Jorge Arístides Miret Riquelme Paola Verónica Arze Gloria Natalia Ramírez Centurión Milena Britos Miriam Rolón

Published in: Revista da Sociedade Brasileira de Medicina Tropical (2022)

This study demonstrated that the use of real-time PCR-HRM using the molecular marker hsp70 was a highly sensitive method for simultaneously detecting and identifying Leishmania species in different tissues taken from infected dogs. In addition, the usefulness of ear pinnae as easily accessible tissue for molecular diagnosis was emphasized.

Keyphrases

real time pcr
gene expression
heat shock protein
single molecule
heat shock
heat stress
oxidative stress
fluorescent probe
molecularly imprinted