Formation of Stem Cell Aggregates and Their Differentiation on Surface-Patterned Hydrogels Based on Poly(2-hydroxyethyl Methacrylate).
Hasani G JayasingheSundararajan V MadihallyYolanda VasquezPublished in: ACS applied bio materials (2019)
Micropillar patterns were fabricated and used to study cell adhesion, morphology, and function. Micropillars were produced in poly(2-hydroxyethyl methacrylate (HEMA)/ N , N -(dimethylaminoethyl)methacrylate (DMAEMA)/tetraethylene glycol dimethacrylate (TEGDMA)) hydrogels using soft lithography, had dimensions of 1 μm diameter, and were either 2.05 or 4.91 μm tall. The patterned hydrogel substrates increased adhesion and induced the formation of cellular aggregates. Digital micrographs were used to quantify aggregate size and number. Differentiation of hMSCs toward adipocytes and chondrocytes was performed using the respective complete culture and differentiation medium for 2 weeks. Cells were stained for Oil red O, Alcian blue, and Type II collagen. Hydrogel substrates supported the differentiation of hMSCs to adipocytes and chondrocytes. The taller micropillar patterns supported the attachment and growth of larger aggregates and were more amenable to aid chondrogenic differentiation.
Keyphrases
- drug delivery
- stem cells
- cell adhesion
- hyaluronic acid
- wound healing
- tissue engineering
- extracellular matrix
- adipose tissue
- induced apoptosis
- mesenchymal stem cells
- escherichia coli
- type diabetes
- signaling pathway
- staphylococcus aureus
- drug release
- cell death
- cell proliferation
- endoplasmic reticulum stress
- bone marrow
- biofilm formation
- gestational age
- high fat diet induced
- preterm birth
- optic nerve