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The 16S rRNA lung microbiome in mechanically ventilated patients: a methodological study.

Melanie FromentinAntoine Bridier-NahmiasJérôme Le GoffSeverine Mercier-DelarueNoémie RangerConstance VuillardJulien Do ValeNoémie ZucmanAntonio AlberdiJean-Damien RicardDamien Roux
Published in: Experimental lung research (2021)
Accuracy of the bacterial lung microbiome composition was highly dependent on the primers used for amplification of the 16 s rRNA hypervariable sequence. This work validates for future lung microbiome studies the use of the 515 F-806R "S-V4" primer pair associated to Illumina® MiSeq paired-end sequencing.
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