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A rapid and efficient DNA extraction method suitable for marine macroalgae.

Gautham Subramaniam RamakrishnanAnwar Aliya FathimaMohandass Ramya
Published in: 3 Biotech (2017)
Macroalgae are a diverse group of organisms. Marine macroalgae, in particular, have numerous medicinal and industrial applications. Molecular studies of macroalgae require suitable concentrations of DNA free of contaminants. At present, numerous protocols exist for DNA extraction from macroalgae. However, they are either time consuming, expensive or work only with few species. The method described in this study is rapid and efficient and applicable to different types of marine macroalgae. This method yields an average of 3.85 µg of DNA per 50 mg of algal tissue, with an average purity of 1.88. The isolated DNA was suitable for PCR amplification of universal plastid region of macroalgae.
Keyphrases
  • circulating tumor
  • single molecule
  • cell free
  • nucleic acid
  • circulating tumor cells
  • risk assessment
  • heavy metals
  • multidrug resistant
  • drinking water
  • wastewater treatment
  • sensitive detection
  • case control
  • gram negative