Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds.
Fatma Zohra ChakouZakaria BoualMohamed Didi Ould El HadjHakim BelkhalfaKhaldoun BachariZainab El Alaoui-TalibiCherkaoui El ModafarFarah HadjkacemImen FendriSlim AbdelkafiMounir TraïkiaDidier Le CerfPascal DubessayDelattre CedricGuillaume PierrePhilippe MichaudPublished in: Plants (Basel, Switzerland) (2021)
The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagi maurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, 1 H/ 13 C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 10 6 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.
Keyphrases
- water soluble
- anti inflammatory
- gas chromatography mass spectrometry
- magnetic resonance
- atomic force microscopy
- high speed
- mass spectrometry
- liver injury
- drug induced
- diabetic rats
- solid phase extraction
- high resolution
- gas chromatography
- tandem mass spectrometry
- single cell
- liquid chromatography
- high performance liquid chromatography
- contrast enhanced
- computed tomography
- endothelial cells
- simultaneous determination