Saccharothrix algeriensis NRRL B-24137, the first non-Streptomyces actinobacterium, produces holomycin after cystine feeding.
Rabiâa MerroucheAmine YekkourYannick CoppelNoureddine BourasAbdelghani ZitouniFlorence MathieuNasserdine SabaouPublished in: Archives of microbiology (2020)
Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. This strain has the ability to produce several dithiolopyrrolone antibiotic derivatives depending on the precursors added to the culture medium. This group of antibiotics is known for their potent antimicrobial and anticancer activities. Holomycin is a member of the dithiolopyrrolone group of antibiotics, and has already been isolated from several species of actinobacteria belonging to the genus Streptomyces and also from some Gram-negative bacteria. In this study, holomycin was produced for the first time in the culture broth of a non-Streptomyces actinobacteria. This antibiotic was induced by adding 5 mM of L-cystine as precursor to the semi-synthetic fermentation broth of Sa. algeriensis NRRL B-24137 and then fully identified after HPLC purification. The minimum inhibitory concentrations (MIC) of holomycin were determined against several pathogenic microorganisms, including Escherichia coli ATCC 10536 Klebsiella pneumoniae CIP 82.91, Listeria monocytogenes CIP 82110, Staphylococcus aureus CIP 7625, Aspergillus carbonarius M333, Fusarium culmorum FC1, Candida albicans IPA 200. This antibiotic showed a broad-spectrum antimicrobial activity, inhibiting a variety of Gram-positive and Gram-negative bacteria, and micro-fungi.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- candida albicans
- staphylococcus aureus
- biofilm formation
- listeria monocytogenes
- multidrug resistant
- gram negative
- ms ms
- signaling pathway
- mass spectrometry
- pseudomonas aeruginosa
- simultaneous determination
- high performance liquid chromatography
- cystic fibrosis
- high resolution
- solid phase extraction
- liquid chromatography
- cell wall