Accurate measurement of extended half-life and unmodified factor VIII low levels with one-stage FVIII assays is dependent on the matrix of calibration curves.
Dorien Van den BosscheJelle ToelenJoke SchoetersIsa Van HorenbeeckIngrid VanlinthoutMirjam DebasseKathelijne PeerlinckMarc G JacqueminPublished in: Haemophilia : the official journal of the World Federation of Hemophilia (2018)
Our data confirm that the generation of calibration curves by dilution in FVIII-deficient plasma is crucial for the accurate measurement of low FVIII levels. When serial dilutions of samples are analyzed, predilution in FVIII-deficient plasma is required to respect the parallelism criteria. These methods should be more generally implemented for coagulation instruments.