Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).
Marine H LaporteNikolai KlenaVirginie HamelPaul GuichardPublished in: Nature methods (2022)
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
Keyphrases
- magnetic resonance
- high resolution
- single molecule
- high speed
- optical coherence tomography
- high throughput
- label free
- living cells
- minimally invasive
- electron microscopy
- healthcare
- room temperature
- single cell
- cell therapy
- computed tomography
- stem cells
- magnetic resonance imaging
- quality improvement
- bone marrow
- silver nanoparticles
- image quality