Live-cell analysis of IMPDH protein levels during yeast colony growth provides insights into the regulation of GTP synthesis.

Erica L ShandKieran SweeneyKaitlin E SundlingMegan N McClean David A Brow

Published in: mBio (2024)

This study used live-cell microscopy to track changes in the level of a key enzyme in GTP nucleotide biosynthesis, inosine monophosphate dehydrogenase (IMPDH), during growth of a brewers yeast colony over 2 days in a microfluidic device. The results show that feedback regulation via transcription attenuation allows cells to adapt to nutrient limitation in the crowded environs of a yeast colony. They also identify a novel mode of regulation of IMPDH level that is not driven by guanine nucleotide availability.

Keyphrases

saccharomyces cerevisiae
cell wall
high throughput
high resolution
single cell
transcription factor
single molecule
cell cycle arrest
optical coherence tomography
circulating tumor cells
cell proliferation
oxidative stress
mass spectrometry
binding protein
high speed