Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions.

James D SwarbrickPhuc UngMatthew L DennisMichael D LeeSandeep ChhabraBim Graham

Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2015)

Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i+4 arrangement within a protein α-helix leads to generation of an EDTA-like metal ion-binding motif. Rigid binding of a Co(II) ion by this motif produces pseudo-contact shifts suitable for paramagnetic NMR structural studies.

Keyphrases

dna binding
binding protein
protein protein
magnetic resonance
fluorescent probe
amino acid
living cells
room temperature
small molecule
gold nanoparticles
solid state
electron transfer