The influence of substrate/inoculum ratio, inoculum source and ammonia inhibition on anaerobic digestion of poultry waste.

Poultry waste are rich in organic matter, allowing its use as substrates for biogas production by anaerobic digestion (AD). The major difficulty in the anaerobic digestion of this protein-rich waste is the ammonia inhibition. Different results of biochemical methane potential (BMP) were obtained after the mesophilic anaerobic digestion of different avian waste in batch mode. It was shown that using two different inoculum (Liger and Saint-Brieuc) sources and different substrate to inoculum (S/I) ratios does not have a significant effect on the biochemical methane potential of organic laying hen droppings (OLHD); an average of 0.272 Nm 3 CH 4 · kg -1 VS was obtained with both inocula. Otherwise, it affects the hydrolysis constant K H , it decreases when substrate to inoculum ratio increases. Furthermore, Liger is the most suitable inoculum for our substrate because it shows a stability during the process even with different organic load. Comparing the biochemical methane potential of multiple avian waste such as organic laying hen droppings and different slaughterhouse waste highlights the importance of slaughterhouse waste in the anaerobic digestion process because of the high methane yield observed specially with the viscera (0.779 Nm 3 CH 4 · kg -1 VS, SD = 0.027 Nm 3 CH 4 · kg -1 VS). Moreover, methane production was affected by increasing the ammonia concentrations; when [N-NH 3 ] > 9.8 g N-NH 3 · L -1 the biochemical methane potential decreases and the lag phase increases (λ > 30 days); a total inhibition of the process was observed when ammonia concentration is above 21.8 g · L -1 .