Linear Peptides Are the Major Products of a Biosynthetic Pathway That Encodes for Cyclic Depsipeptides.
Thomas P WycheAntonio C RuzziniChristine BeemelmannsKi Hyun KimJonathan L KlassenShugeng CaoMichael PoulsenTim S BugniCameron R CurrieJon ClardyPublished in: Organic letters (2017)
Three new dentigerumycin analogues are produced by Streptomyces sp. M41, a bacterium isolated from a South African termite, Macrotermes natalensis. The structures of the complex nonribosomal peptide synthetase-polyketide synthase (NRPS/PKS) hybrid compounds were determined by 1D- and 2D-NMR spectroscopy, high-resolution mass spectrometry, and circular dichroism (CD) spectroscopy. Both cyclic and linear peptides are reported, and the genetic organization of the NRPS modules within the biosynthetic gene cluster accounts for the observed structural diversity.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- genome wide
- high resolution
- copy number
- ultra high performance liquid chromatography
- gas chromatography
- amino acid
- mass spectrometry
- tandem mass spectrometry
- single molecule
- molecular docking
- dna methylation
- genome wide identification
- gene expression
- solid state
- network analysis
- ms ms