Bioactive ambuic acid congeners from endophytic fungus Pestalotiopsis trachicarpicola SC-J551.
Fanyu DongZhiming JiangPing WuFangfang DuanJinghua XueHaibo TanXiao-Yi WeiPublished in: The Journal of antibiotics (2023)
New ambuic acid derivatives, pestallic acids R-V (1-5), together with ambuic acid (6), were isolated from the endophytic fungus Pestalotiopsis trachicarpicola SC-J551 derived from the fern Blechnum orientale L., of which compound 2, being racemic, was separated to two optically pure enantiomers (+)-2 and (-)-2. The structures including absolute configurations of these new compounds were elucidated by extensive spectroscopic analysis and theoretical simulations of their ECD spectra and 13 C NMR chemical shifts. Compounds 1 and 3 exhibited cytotoxicity against human carcinoma A549, HeLa, HepG2, and MCF-7 cells (IC 50 : 3.6-12.5 μM) and compound 3 was also active against Staphylococcus aureus and MRSA (MIC = 20 μg ml -1 ). Compound (±)-2 showed inhibitory activity against LPS-induced NO release (IC 50 = 21.1 μM) and t-BHP-induced ROS production (IC 50 = 8.5 μM) in RAW264.7 macrophages.
Keyphrases
- staphylococcus aureus
- lps induced
- inflammatory response
- cell cycle arrest
- high resolution
- endothelial cells
- cell death
- induced apoptosis
- magnetic resonance
- high glucose
- molecular docking
- breast cancer cells
- escherichia coli
- signaling pathway
- induced pluripotent stem cells
- molecular dynamics simulations
- pi k akt
- endoplasmic reticulum stress
- capillary electrophoresis
- plant growth