Nontarget Screening and Toxicity Evaluation of Diol Esters of Okadaic Acid and Dinophysistoxins Reveal Intraspecies Difference of Prorocentrum lima.
Haiyan WuJiaqi ChenJixing PengYun ZhongGuanchao ZhengMengmeng GuoZhijun TanYuxiu ZhaiSonghui LuPublished in: Environmental science & technology (2020)
High-resolution mass spectrometry (HRMS) analysis with the assistance of molecular networking was used to investigate intracellular toxin profiles of five Prorocentrum lima (P. lima) strains sampled from the north Yellow Sea and South China Sea. Mice were used as a model species for testing the acute toxicity of intracellular okadaic acid (OA) and dinophysistoxins (DTXs) in free and esterified states. Results showed that OA and DTX1 esterified derivatives were detected in all P. lima samples, accounting for 55%-96% of total toxins in five strains. A total of 24 esters and 1 stereoisomer of DTX1 (35S DTX1) were identified based on molecular networking and MS data analysis, 15 esters of which have been reported first. All P. lima strains displayed specific toxin profiles, and preliminary analysis suggested that toxin profiles of the five P. lima strains might be region-related. Moreover, acute toxicity in mice suggested higher toxicity of esters compared with free toxins, which highlights the importance and urgency of attention to esterified toxins in P. lima.
Keyphrases
- escherichia coli
- high resolution mass spectrometry
- data analysis
- oxidative stress
- liver failure
- liquid chromatography
- mass spectrometry
- respiratory failure
- drug induced
- high fat diet induced
- ms ms
- working memory
- type diabetes
- single molecule
- oxide nanoparticles
- single cell
- genome wide
- skeletal muscle
- extracorporeal membrane oxygenation
- tertiary care