Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale.

Manuel F Juette Daniel S TerryMichael R WassermanRoger B AltmanZhou ZhouHong ZhaoScott C Blanchard

Published in: Nature methods (2016)

Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.

Keyphrases

single molecule
high resolution
living cells
atomic force microscopy
high throughput
climate change
ionic liquid