According to acculturative family distancing theory, adolescents' perceptions of cultural incongruencies with parents can diminish the quality of parent-adolescent relationships and, as a result, harm adolescent adjustment. Using four time points of data for a sample of 547 diverse Latino/a/x adolescents, this study examined how parent-adolescent relationship quality and acculturative family distancing were associated with changes in adolescent school performance and internalizing symptoms. At baseline, the school-based sample ranged from 11- to 14-years-old ( M = 12.78) and included slightly more females (55%) than males (45%). Cross-lagged structural equation model results indicated that adolescent reports of greater acculturative family distancing were associated with adolescent perceived increases in parent-adolescent conflict and decreases in parental support. Conflict mediated associations between acculturative family distancing and decreased school performance. Associations between parent-child relationship qualities and Latino/a/x adolescent adjustment were bidirectional.