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Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.

F Phil BrooksHunter C DavisPojeong ParkYitong QiAdam E Cohen
Published in: bioRxiv : the preprint server for biology (2024)
Voltage sensitivity in FRET-opsin indicators comes from a photocycle intermediate, reachable via optimized 2P excitation.
Keyphrases
  • living cells
  • energy transfer
  • single molecule
  • fluorescent probe
  • high resolution
  • quantum dots
  • fluorescence imaging