The guide RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.

Published in: bioRxiv : the preprint server for biology (2024)

• Sequence of guide RNA can alter slicing rate of fully paired substrate by 250-fold• Sequences that cause more rapid slicing direct more efficient RNAi in cells• Strong central pairing imparts tolerance for mismatches to the guide 3' region• This tolerance is attributable to more fully populating the slicing conformation.

Keyphrases

induced apoptosis
molecular dynamics simulations
amino acid
cell cycle arrest

molecular dynamics
nucleic acid
single molecule

signaling pathway
oxidative stress
endoplasmic reticulum stress

cell death
crystal structure
quantum dots