The southern root-knot nematode (RKN), Meloidogyne incognita, is the most disastrous and prevalent nematode threat to the production of crops, especially vegetables. In the current study, second-stage juveniles (J2) of M. incognita were collected from five regions near Tai'an, China. The toxicity of abamectin to these J2 had insignificant differences, with LC50 values of approximately 2 mg/L. Two pesticide application approaches (i.e., blending-of-soil and root-irrigation) were adopted in pot experiments; blending-of-soil was more beneficial for promoting the efficacy of abamectin on the RKN of tomatoes. Abamectin microcapsule suspension exhibited superiority to emulsifiable concentrate (EC) at dosages of 5 and 10 mg active ingredient per plant integrating efficacy, root length, plant height, the fresh weight of roots, and the fresh weight of stems + leaves. Adsorption, leaching, and mobility of abamectin in the soil also verified bioactivity test results. Modifying the formulation of abamectin can promote its efficacy on RKN under different application approaches.