Internalization and accumulation of model lignin breakdown products in bacteria and fungi.
Meghan C Barnhart-DaileyDongmei YeDulce C HayesDanae MaesCasey T SimoesLeah AppelhansAmanda Carroll-PortilloMichael S KentJerilyn A TimlinPublished in: Biotechnology for biofuels (2019)
Mass spectrometry and single-cell microscopy methods were developed to establish a toolset for providing direct measurement and visualization of relative internal concentrations of mono- and di-aryl compounds in microbes. Utilizing these methods, we observed broad variation in intracellular concentration between organisms and within populations and this may have important consequences for the efficiency and productivity of an industrial process for bioconversion. Subsequent application of this toolset will be useful in identifying and characterizing specific transporters for lignin-derived mono- and di-aryl compounds.
Keyphrases
- mass spectrometry
- single cell
- high resolution
- ionic liquid
- high throughput
- biofilm formation
- rna seq
- liquid chromatography
- climate change
- single molecule
- heavy metals
- optical coherence tomography
- high speed
- high performance liquid chromatography
- reactive oxygen species
- capillary electrophoresis
- gram negative
- escherichia coli
- staphylococcus aureus
- cystic fibrosis
- multidrug resistant
- tandem mass spectrometry