Enhanced Immune Responses to Mucosa by Functionalized Chitosan-Based Composite Nanoparticles as a Vaccine Adjuvant for Intranasal Delivery.
Yuhong LinBeini SunZheng JinKai ZhaoPublished in: ACS applied materials & interfaces (2022)
Nasal administration for vaccine delivery is a novel non-invasive vaccine administration approach that can induce local or systemic immune responses and overcome the disadvantages caused by traditional injectable administration. However, mucosal vaccine and adjuvant delivery systems with sustained-release ability and enhanced immune effects at mucosal sites have still been highly demanded. In this work, N -2-hydroxypropyl trimethyl ammonium chloride chitosan/ N , O -carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) with excellent mucosal absorption, high drug loading capacity, and enhanced immune responses were prepared by the ionic cross-linking method. To evaluate the potential capacity of the N-2-HACC/CMCS NPs as a vaccine adjuvant and the molecular mechanism for the induction of enhanced mucosal and systemic immune responses, bovine serum albumin (BSA) was employed as a general model antigen and loaded into the N-2-HACC/CMCS NPs to prepare a BSA-loaded N-2-HACC/CMCS adjuvant vaccine (N-2-HACC/CMCS/BSA NPs). It was well demonstrated that the N-2-HACC/CMCS/BSA NPs with great biostability and mucosal absorption could effectively promote the proliferation of lymphocytes and the secretion of related pro-inflammatory factors, resulting in the stimulation of specific mucosal and systemic immune responses. This study revealed that the chitosan-based nano-delivery system can act as the mucosal vaccine adjuvant and possesses great promise in viral infectious diseases and immunization therapy.
Keyphrases
- immune response
- drug delivery
- early stage
- ulcerative colitis
- wound healing
- toll like receptor
- dendritic cells
- infectious diseases
- hyaluronic acid
- cancer therapy
- signaling pathway
- emergency department
- sars cov
- mesenchymal stem cells
- ionic liquid
- machine learning
- peripheral blood
- mass spectrometry
- inflammatory response
- drug induced
- climate change
- artificial intelligence
- bone marrow
- tandem mass spectrometry